1,25(OH)2 VITAMIN D, RIA CT, 48 TESTS

The DIAsource 1,25(OH)2 Vitamin D RIA is a competitive RIA assay, based on the Gold Standard extraction methodology.

The assay shows equal or superior sensitivity, precision and cross-reactivity profile to all other RIA assays in the market.

Only samples and controls, not the calibrators, are extracted with a mix of solvents and applied on cartridges to separate 1,25(OH)2 Vitamin-D from other Vitamin-D metabolites. After elution of samples and controls, the calibrators, samples and controls are incubated in coated tubes. A fixed amount of 125I labelled 1,25(OH)2 Vitamin D competes with the 1,25(OH)2 Vitamin D to be measured present in the sample or in the calibrator for a fixed amount of antibody sites immobilized on the wall of a polystyrene tube. After an overnight incubation at room temperature, an aspiration step terminates the competition reaction. The tubes are then washed with washing solution and aspirated. A calibration curve is plotted and the 1,25(OH)2 Vitamin D concentrations of the samples are determined by dose interpolation from the calibration curve.

Catalog #: KIP1929
Format: RIA CT
Label: I125
Size: 48 tests
Sample Type: Serum, Plasma
Sample Volume: 500 µL
Controls: 2 levels
Range: 4.7-411 pg/mL
Sensitivity: 1.6 pg/mL
Incubation: Overnight
Shelf Life (weeks): 10

https://www.diasource-diagnostics.com/IVD-Products/ImmunoAssays/Bone-Metabolism/Vitamin-D/1-25-OH-2-Vitamin-D-RIA-CT-48-tests

Research Use Only.