3-CAT, RIA, 3 X 96 TESTS

Adrenaline (epinephrine), noradrenaline (norepinephrine) and dopamine are extracted by using a cis-diol- specific affinity gel, acylated and then converted enzymatically. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.

Catalog #: KIPL1600
Format: RIA
Label: I125
Size: 3 x 96 tests
Sample Type: EDTA Plasma, Urine
Sample Volume: 600/15 µL
Controls: 2 levels
Range: see Adrenaline, Noradrenaline and Dopamine RIA
Sensitivity: see Adrenaline, Noradrenaline and Dopamine RIA
Incubation: see Adrenaline, Noradrenaline and Dopamine RIA
Shelf Life (weeks): 14