ANGIOTENSIN I RIA, RIA CT, 100 TESTS

The Angiotensin I RIA serves for the quantitative determination of plasma renin activity (PRA) by the radioimmunoassay of the product of the reaction, angiotensin I. The generation of angiotensin I is the result of the enzymatic cleavage of the renin substrate, angiotensinogen, in plasma samples in the presence of ACE inhibitor (ACE - Angiotensin-Converting Enzyme), an enzymatic inhibitor that blocks the conversion of angiotensin I to angiotensin II. The immunoassay of angiotensin I is a radioimmunological competition assay. Unknown samples, control, and calibrators are incubated in polyclonal antibody-coated tubes with 125I-labeled angiotensin I as tracer. After incubation, the contents of the tubes are aspirated. The bound radioactivity is then determined in a gamma counter. A standard curve is established and unknown values are determined by interpolation from the standard curve.

Catalog #: KIPB3518
Format: RIA CT
Label: I125
Size: 100 tests
Sample Type: Plasma
Sample Volume: 400 µL
Controls: 1 level
Range: 0.30 - 30.0 ng/mL
Sensitivity: 0,07 ng/mL
Incubation: 2 h RT with shaking at 18-25°C
Shelf Life (weeks): 8