NuProbe

CNV+ Breast Cancer NGS Panel

CNV-NGS-Panel

Contact us for more information.

An NGS panel for detection of mutation hotspots in 14 genes and copy number variations in 18 genes associated with breast cancer
  • ULTRASENSITIVE: Detect mutations at 0.2% VAF and deletions at 1.94 ploidy
  • QUANTITATIVE: Quantitate mutation VAF of samples down to 0.2% VAF
  • QUICK TURNAROUND TIME: 6-hour DNA-to-Library workflow
  • VERSATILE: Compatible with several sample types
  • USER-FRIENDLY: Easy workflow and data analysis

For Research Use Only

CNV+ Breast Cancer NGS Panel:

The CNV+ Breast Cancer NGS Panel covers 84 frequently mutated cancer hotspots in 14 genes and CNVs of 18 genes including HER2, amplified in 10-20% of breast cancers and guide treatment with trastuzumab (HERCEPTIN).

The CNV+ Breast Cancer NGS Panel utilizes unique molecular identifiers(UMI) and PCR to accurately quantify SNVs and Indels down to 0.2% VAF as well as detect CNVs in HER2 above 2.04 copies and below 1.97 copies from cfDNA.

Unlike FISH, the CNV+ Breast Cancer NGS Panel can detect hotspot mutations in 14 genes and HER2 CNVs simultaneously in the same reaction. Amplicons all along HER2 allow for ultrafine CNV mapping with sub-gene level identification of CNVs.

The CNV+ Breast Cancer NGS Panel has a high conversion yield (median >60%) allowing sensitive detection from small sample inputs (30ng FFPE DNA, 8ng fresh/frozen DNA, 10ng cfDNA).

Benefits:

  • CNV and Hotspots: covers 84 mutation hotspots in 14 genes and detect Copy Number Variations (CNVs) in 18 genes including ERBB2 (HER2)
  • Ultrasensitive: SNV and Indels detected and quantitated down to 0.2% VAF; CNVs (amplifications and deletions) detected and quantitated at ≥2.04 and ≤1.97 copies (amplifications and deletions)
  • Sample Flexibility: compatible with several sample types, including cfDNA, tumor section (FFPE or Fresh Frozen), and PBMC DNA. Need as little as 10 ng of DNA
  • Calibration-free quantitation: high quantitation accuracy of variants down to 0.2% VAF
  • Dedicated data analysis pipeline: fastq-to-variant report with a user-friendly cloud or locally-run analysis program

Hotspot Mutations:

AKT1 AR BRAF BRCA1 BRCA2
EGFR ERBB2 ERBB3 ERBB4 ESR1
KRAS PIK3CA PTEN TP53

CNVs:

AKT3 BRCA1 BRCA2 BRIP1 EGFR ERBB2
ERBB3 ERBB4 ESR1 FGFR1 MYC NBN
NCSTN PIK3CA PTEN PTGS2 RAD51C TP53

 

NuProbe Product Page