VarTrace® EGFR Assays


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Targeted qPCR-based assays to identify mutations down to 0.1% VAF

  • SENSITIVE: Limit of detection down to 0.1% VAF

  • QUANTITATIVE: Able to quantify mutation VAF in original sample

  • VERSATILE: Applicable to a variety of sample types

  • COMPREHENSIVE: Wide range of mutations covered

  • USER-FRIENDLY: Easy workflow

For research use only. Not for use in diagnostic procedures.

The VarTrace products enable highly sensitive detection and quantification of mutations for NSCLC, AML, melanoma patients. The kits use NuProbe’s PCR-based Blocker Displacement Amplification (BDA) technology to enable the selective amplification of low abundant sequence variants (SNV and indels) in a background of wildtype DNA. Following PCR enrichment, Sanger sequencing is applied to reveal the identity of the variants.


  • Detects 272 EGFR mutations and quantitates 43 mutations
  • Enables distinction of cis/trans mutations in C797S/T790M
  • Compatible with plasma samples, tumor biopsies, paraffin-embedded sections (FFPE), and fresh frozen tumors
  • Accepts 10-100 ng cfDNA from blood plasma, or 10-1000 ng DNA from tissue biopsy
  • Accepts 2-10 ml of blood, 1-5ml of blood plasma, as little as 1mm*1mm (5 μm) tissue sections (fresh frozen or FFPE)
  • Delivers results in 8 hours, with 2.5 hours of hands-on time

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