CytoTest LSP CCND1 3' FISH ProbeCT-LSP122
The CCND1 3’ FISH Probe is designed to detect the human CCND1 gene 3’-region located on chromosome band 11q13.3. CCND1 is also known as BCL1, PRAD1, U21B31 or D11S287E.
The CCND1 3' FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.
The CCND1 3' FISH probe hybridizes to chromosome 11 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 11q13.3 region on the chromosome 11. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 11q13.3. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 11.
For Research Use Only.