LSP D22S75 FISH Probe

The D22S75 FISH Probe is designed to detect the human D22S75 (N25) region located on chromosome band 22q11.21.

The D22S75 FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

The D22S75 FISH probe hybridizes to chromosome 22 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 22q11.21 region on the chromosome 22.  In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 22q11.21.  Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 22.

for Research use Only.