CytoTest LSP D5S23, D5S721 FISH ProbeCT-LSP053
The D5S23, D5S721 FISH Probe is designed to detect the human D5S23, D5S721 region located on chromosome band 5p15.2.
The D5S23, D5S721 FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, giving it an orange fluorescent signal with the appropriate filters.
The D5S23, D5S721 FISH probe hybridizes to chromosome 5 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct green fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 5p15.2 region on the chromosome 5. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 5p15.2. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 5.
For Research Use Only.