CytoTest LSP EGFR FISH ProbeCT-LSP006
The EGFR FISH Probe is designed to detect the human EGFR gene located on chromosome band 7p11.2. EGFR is also known as TIS8, AT225, G0S30, NGFI-A, ZNF225, KROX-24 or ZIF-268.
The EGFR FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.
The EGFR FISH probe hybridizes to chromosome 7 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 7p11.2 region on the chromosome 7. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 7p11.2. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 7.
For Research Use Only.