CytoTest LSP IGH 3' FISH ProbeCT-LSP114
The IGH 3’ FISH Probe is designed to detect the human IGH locus 3’-region located on chromosome band 14q32.33.
The IGH 3' FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, giving it a green fluorescent signal with the appropriate filters.
The IGH 3' FISH probe hybridizes to chromosome 14 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 14q32.33 region on the chromosome 14. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 14q32.33. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 14.
For Research Use Only.