D-Tek BlueDot ANA12 Screen IgG Kit
D-Tek

D-Tek BlueDot ANA12 Screen IgG Kit

ANA12SD-24

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BlueDOT ANA12 Screen IgG is an Immunodot kit intended for the detection in human sera of IgG autoantibodies against Sm, RNP(68kD /A/C), Sm/RNP, SSA(Ro), SSB(La), Jo-1(histidyl-t-RNA synthetase), Scl-70(DNA topoisomerase I), PM-Scl, Ku, CENP-A/B(centromere A + B proteins), PCNA and Mi-2 antigens. Each kit contains 24 tests.

ANA12_Screen_IgG_Protocol.pdf ANA12_Screen_IgG_Protocol.pdf

BlueDot kits are composed of 24 nitrocellulose strips on which the antigens have been coated in a dot-shaped format. One strip allows the testing of one patient for many different autoantibodies in the same run. The test is based on a classical Enzyme ImmunoAssays (EIA).

In addition to the antigens, each strip possesses two built-in reactive controls :

  • The first one, the Reaction Control (RC), controls the validity of the test : its coloration proves that the clinician has added the patient serum and that the test has functioned correctly.
  • The second one, the Cut-Off Control (CO), allows the interpretation of the test : any color intensity equal to or below that of the cut-off is considered negative, any color intensity superior to that of the cut-off is considered positive.

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The results can be interpretated by sight or a semi-quantification can be obtained thanks to the Dr DOT software.