D-Tek BlueDot Liver10 IgG Kit
D-Tek

D-Tek BlueDot Liver10 IgG Kit

LI10D-24

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BlueDOT Liver10 IgG is an Immunodot kit intended for the detection in human sera of IgG autoantibodies against M2/native PDC (E1, E2, E3 subunits of Pyruvate Dehydrogenase Complex), M2/OGDC-E2 (Subunit E2 of OxoGlutarate Dehydrogenase Complex), M2/BCOADC-E2 (Subunit E2 of Branched-Chain Oxo Acid Dehydrogenase Complex) and M2/PDC-E2 (Subunit E2 of Pyruvate Dehydrogenase Complex), gp210 (Nuclear Membrane associated antigen), sp100 (Multiple Nuclear Dots associated antigen), LKM1 (P450 2D6 fusion tripeptide), LC1 (Formiminotransferase Cyclodeaminase), SLA (Soluble Liver Antigen) and F-Actin antigens. Each kit contains 24 tests.

Liver10_IgG_Protocol.pdf Liver10_IgG_Protocol.pdf

BlueDot kits are composed of 24 nitrocellulose strips on which the antigens have been coated in a dot-shaped format. One strip allows the testing of one patient for many different autoantibodies in the same run. The test is based on a classical Enzyme ImmunoAssays (EIA).

In addition to the antigens, each strip possesses two built-in reactive controls :

  • The first one, the Reaction Control (RC), controls the validity of the test : its coloration proves that the clinician has added the patient serum and that the test has functioned correctly.
  • The second one, the Cut-Off Control (CO), allows the interpretation of the test : any color intensity equal to or below that of the cut-off is considered negative, any color intensity superior to that of the cut-off is considered positive.

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The results can be interpretated by sight or a semi-quantification can be obtained thanks to the Dr DOT software.