PathoNostics
Dermatophytes represent the most numerous and widespread group of all mycoses and have the capacity to invade keratinized tissue of humans and animals to facilitate infections that are generally restricted to the corneocytes of the skin, hair and nails.
DermaGenius® 2.0/3.0 Complete Multiplex real-time PCR kit
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Dermatophytes represent the most numerous and widespread group of all mycoses and have the capacity to invade keratinized tissue of humans and animals to facilitate infections that are generally restricted to the corneocytes of the skin, hair and nails.
Dermatophytes are classified in three genera: Epidermophyton, Microsporum and Trichophyton. Diagnosis of these infections is currently based on microscopy or histology associated with culture on specific agar media. However, direct microscopy lacks specificity and culturing has a long turn-around-time of 2-4 weeks and is prone to contamination. These limitations can be prevented by the use of molecular diagnostics.
Targets:
- Trichophyton rubrum / Trichophyton soudanense
- Trichophyton interdigitale / Trichophyton mentagrophytes
- Trichophyton tonsurans
- Trichophyton schoenleinii / Trichophyton quinckeanum
- Trichophyton violaceum
- Trichophyton benhamiae
- Trichophyton verrucosum
- Microsporum canis
- Microsporum audouinii
- Nannizzia gypsea*
- Epidermophyton floccosum
- Candida albicans
- Candida parapsilosis*
- Scopulariopsis brevicaulis*
- Pan-dermatophyte*
- Internal control
Features and benefits:
- Aids in the diagnosis of dermatophytosis (superficial fungal infections)
- Detects > 95% of the clinically most relevant dermatophytes
- Dermatophyte species identification, enabling: Targeted Treatment
- Detection of mutations in the squalene epoxidase (SQLE) gene (D. Resistance)
- Differentiation between wildtype and resistant SQLE strains (D. Resistance)
- Detection in nail, skin and hair samples
- Sample-to-result in less than 3 hours
- Higher sensitivity: up to 20% more positive results compared to culture
- Identification of source of infection
- Assistance in outbreak management
- Including Pan-dermatophyte mix
Real-time PCR instruments:
- LightCycler 480 II (Roche)
- Rotor-Gene Q (QIAGEN)
- CFX96 (Bio-rad)
- QuantStudio 5 (Thermo Fisher Scientific)
- Mic qPCR cycler (Bio Molecular Systems)
Quality:
- Validated on clinical samples
- Internal control (IC) included
- Positive and negative controls (PCs) included
- Validated on EQA programmes of QCMD