DermaGenius® 3.0 Nail Multiplex real-time PCR kit

Dermatophytes represent the most numerous and widespread group of all mycoses and have the capacity to invade keratinized tissue of humans and animals to facilitate infections that are generally restricted to the corneocytes of the skin, hair and nails.
Dermatophytes are classified in three genera: Epidermophyton, Microsporum and Trichophyton. Diagnosis of these infections is currently based on microscopy or histology associated with culture on specific agar media. However, direct microscopy lacks specificity and culturing has a long turn-around-time of 2-4 weeks and is prone to contamination. These limitations can be prevented by the use of molecular diagnostics.

Targets:

• Trichophyton rubrum
• Trichophyton interdigitale
• Candida albicans
• Candida parapsilosis
• Scopulariopsis brevicaulis
• Internal control

Features and benefits:

• Aids in the diagnosis of dermatophytosis (superficial fungal infections)
• Detects > 95% of the clinically most relevant dermatophytes
• Dermatophyte species identification, enabling: Targeted Treatment
• Detection of mutations in the squalene epoxidase (SQLE) gene (D. Resistance)
• Differentiation between wildtype and resistant SQLE strains (D. Resistance)
• Detection in nail, skin and hair samples
• Sample-to-result in less than 3 hours
• Higher sensitivity: up to 20% more positive results compared to culture
• Identification of source of infection
• Assistance in outbreak management
• Including Pan-dermatophyte mix

Real-time PCR instruments:

• LightCycler 480 II (Roche)
• Rotor-Gene Q (QIAGEN)
• CFX96 (Bio-rad)
• QuantStudio 5 (Thermo Fisher Scientific)
• Mic qPCR cycler (Bio Molecular Systems)

Quality:

• Validated on clinical samples
• Internal control (IC) included
• Positive and negative controls (PCs) included
  
• Validated on EQA programmes of QCMD
  

Product PDF