SEROTONIN, RIA, 100 TESTS

First, Serotonin is quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125I-labelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a reference curve prepared with known standards.

Catalog #: KIPL0900
Format: RIA
Label: I125
Size: 100 tests
Sample Type: See Remarks
Sample Volume: 25 µL
Controls: 2 levels
Range: 20-2000 ng/mL
Sensitivity: 0,33 to 6,7 ng/mL
Incubation: 2,5 hours at 2-8°C
Shelf Life (weeks): 14