SIV Blot (30)

Nitrocellulose strips contain SIVMAC viral proteins resulting from electrophoresis and electroblotting of detergent disrupted virus particles.(8,9)A serum control band is included on each strip to minimize the risk of false negative results due to operational error. Individual strips are reacted with diluted serum or plasma specimens. If specific SIV antibodies are present, they will bind to the SIV proteins on the nitrocellulose. The strips are washed to remove unbound material. The bound antibodies are visualized by subsequent reaction with goat anti-human IgG conjugated with alkaline phosphatase, followed by BCIP/NBT substrate.