25OH VITAMIN D3, RIA CT, 96 TESTS

The DIAsource 25OH Vitamin D3 RIA is a competitive RIA assay, specific for the D3 form of 25OH Vitamin D.

The assay is calibrated to the ID-LC-MS/MS reference method developed by Prof. Dr. Linda THIENPONT from the Ghent University in Belgium, as encompassed in the Vitamin D Standardization Program initiative. It shows a high correlation with other commercially available LC-MS/MS and RIA assays.

At first calibrators, controls and samples (serum or plasma) are extracted with acetonitrile. A fixed amount of 125I labelled 25OH Vitamin D3 competes with the 25OH Vitamin D3 from either extracted samples, controls or calibrators for a fixed amount of specific antibody sites immobilized to the lower and inner surface of plastic tubes.

After 2 hours incubation at room temperature, an aspiration step stops the competition reaction. The tubes are then washed with 3 ml washing solution and counted in a gamma counter.

Catalog #: KIP1961
Format: RIA CT
Label: I125
Size: 96 tests
Sample Type: Serum
Sample Volume: 200 µL
Controls: 2 levels
Range: 3-130 ng/mL
Sensitivity: 1,2 ng/mL
Incubation: 2 hours at room temperature
Shelf Life (weeks): 10