Ampli1™ WGA Kit

Balanced, robust whole genome amplification from single cells

The Ampli1™ Whole Genome Amplification (WGA) kit has been developed and optimized for balanced and complete amplification of the total DNA content of a single cell. The output of the Ampli1 WGA protocol is a well-balanced library of approximately 19 million fragments representing the entire genome.  This DNA library is ideally suited for downstream analysis of both SNPs and CNV in single cells, due to its low rate of preferential amplification.

Single tube protocol ideal for all sample types

The Ampli1 WGA Kit is designed to provide optimal whole genome amplification—not only from samples containing DNA from a few cells, but from DNA obtained from one single cell. Input DNA may be derived from fresh, fixed, and/or stained cells.

Following cell lysis, DNA is digested with a restriction enzyme and adaptors are ligated onto the DNA fragments. Amplification, performed in the same tube, is mediated by a single highly specific PCR primer for all 19 million fragments.

Reliable, Reproducible, Balanced Single Cell Amplification

Key features of the Ampli1 WGA Kit:

• Single-tube, no-precipitation protocol minimizes template loss;
• Single, highly specific primer-mediated PCR ensures balanced amplification of both alleles;
• Robust protocol generally produces about 2 micrograms dsDNA and 5 micrograms of ssDNA.

Amplified DNA may be used in a variety of downstream applications, including:

• Sanger Sequencing
• Next-generation sequencing (NGS) - with Ampli1 LowPass and Ampli1 OncoSeek Panel
• Microsatellite analysis
• Copy number variation (CNV) analysis by RT-PCR or aCGH