Ampli1™ QC KitAmpli1-QC-Kit
The Ampli1™ QC Kit provides a useful quality control check of the WGA procedure by utilizing a PCR-based assay to establish DNA integrity.
By testing for the presence of one short and three long DNA fragments in the WGA library, the quality of the DNA as starting material for downstream analyses can be assured.
Cells with good quality DNA typically produce 3 or 4 PCR bands, while fixation of cells or cells with degraded DNA will show fewer QC bands. It has been proved that the number of the bands revealed is useful to define a “Genome Integrity Index” (GII) for predicting success of various downstream analytical methods and for assessing the quality of the starting DNA.