CytoTest LSP BCR FISH ProbeCT-LSP178
The BCR FISH Probe is designed to detect the human BCR gene located on chromosome band 22q11.23. BCR is also known as BCR1 or PHL.
The BCR FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, giving it an green fluorescent signal with the appropriate filters.
The BCR FISH probe hybridizes to chromosome 22 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 22q11.23 region on the chromosome 22. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 22q11.23. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 22.
For Research Use Only.