LSP CDKN2A FISH Probe

The CDKN2A FISH Probe is designed to detect the human CDKN2A gene located on chromosome band 9p21.3. CDKN2A is also known as ARF, MLM,P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A or P16-INK4A.

The CDKN2A FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

The CDKN2A FISH probe hybridizes to chromosome 9 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 9p21.3 region on the chromosome 9.  In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 9p21.3.  Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 9.

For Research Use Only.