LSP D13S319 FISH Probe

The D13S319 FISH Probe is designed to detect the human D13S319 region located on chromosome band 13q14.2. 

The D13S319 FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

The D13S319 FISH probe hybridizes to chromosome 13 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 13q14.2 region on the chromosome 13.  In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 13q14.2.  Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 13.