LSP DDIT3 5 FISH Probe

The DDIT3 5’ FISH Probe is designed to detect the human DDIT3 gene 5’-region located on chromosome band 12q13.3. DDIT3 is also known as CHOP, CEBPZ, CHOP10, CHOP-10, GADD153.

The DDIT3 5 FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, giving it a green fluorescent signal with the appropriate filters.

The DDIT3 5 FISH probe hybridizes to chromosome 12 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 12q13.3 region on the chromosome 12. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 12q13.3. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 12.

For Research Use Only.