CytoTest LSP ERG 3' FISH Probe

CytoTest LSP ERG 3' FISH Probe


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The ERG 3' FISH Probe is designed to detect the human ERG gene 3’-region located on chromosome band 21q22.2. ERG is also known as p55 or erg-3.

The ERG 3' FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.

ERG 3' FISH probe hybridizes to chromosome 21 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 21q22.2 region on the chromosome 21. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 21q22.2. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 21.

For Reseaerch Use Only.