CytoTest LSP FHIT FISH ProbeCT-LSP018
The FHIT FISH Probe is designed to detect the human FHIT gene located on chromosome band 3p14.2. FHIT is also known as FRA3B or AP3Aase.
The FHIT FISH probe is labeled with CytoOrange. CytoOrange is a fluorophore with an excitation peak at 551nm and emission peak at 575nm, giving it an orange fluorescent signal with the appropriate filters.
The FHIT FISH probe hybridizes to chromosome 3 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct orange fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 3p14.2 region on the chromosome 3. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 17q 3p14.2 2. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 3.
For Research Use Only.