LSP KMT2A 3 FISH Probe

The KMT2A 3’ FISH Probe is designed to detect the human KMT2A gene 3’-region located on chromosome band 11q23.3. KMT2A is also known as HRX, MLL, MLL1, TRX1, ALL-1, CXXC7, HTRX1, MLL1A, WDSTS, MLL/GAS7 or TET1-MLL.

The KMT2A 3 FISH probe is labeled with CytoGreen. CytoGreen is a fluorophore with an excitation peak at 495nm and emission peak at 518nm, giving it a green fluorescent signal with the appropriate filters.

The KMT2A 3 FISH probe hybridizes to chromosome 11 in both metaphase and interphase cells. After hybridizing with normal human peripheral blood lymphocyte samples, two distinct fluorescent spots are observed in the interphase nuclei, each spot corresponding to the 11q23.3 region on the chromosome 11. In metaphase cells, a “doublet” or “split signal” may appear on chromosomal region 11q23.3. Each signal within the doublet represents one of the two sister chromatids present in the replicated chromosome 11.

For Research Use Only.