PROtrol™ SARS-CoV-2 Lineage B.1.1.529; Omicron Variant (Isolate: USA/MD-HP20874/2021) (1 mL)

Description:

SARS-Related Coronavirus 2 Lineage B.1.1.529; Omicron Variant (Isolate: USA/MD-HP20874/2021) is an enveloped, positive-sense single-stranded RNA virus from the Coronaviridae family and the Betacoronaviridae genus. Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA/MD-HP20874/2021 was isolated from a nasal swab from a human as part of surveillance testing, on November 27, 2021 in Maryland, USA. Under the nomenclature system introduced by GISAID (Global Initiative on Sharing All Influenza Data), SARS-CoV-2, isolate USA/MD- HP20874/2021 is assigned lineage B.1.1.529 and GISAID clade GR using Phylogenetic Assignment of Named Global Outbreak LINeages (PANGOLIN) tool.

SARS-CoV-2 was propagated in VeroE6 cells. The viral culture fluid was clarified via centrifugation to remove cellular debris prior to inactivation.

PROtrol™ (Protein control) is formulated with viral particles that have been modified to render them non-infectious. Each vial contains 1.0 mL of SARS- CoV-2 PROtrol™ frozen in 2% MEM media.

The pre-inactivation titer was determined from an infectious aliquot. Each lot of PROtrol™ is tested using an N-protein ELISA developed internally at ZeptoMetrix.

Viral inactivation is verified by the absence of viral growth in tissue culture-based infectivity assay.